Journal: Pharmacological research

Article Title: CircRNA CDR1as affects functional repair after spinal cord injury and regulates fibrosis through the SMAD pathway.

doi: 10.1016/j.phrs.2024.107189

Figure Lengend Snippet: Fig. 4. CDR1as regulates NSCs differentiation and microglia polarization. NSCs differentiation was identified by immunofluorescence. GFAP staining was used to detect astrocytes and MAP2 was used to detect nerve cells (A-B). The differentiation was further identified by PCR. The characteristics of nerve cells included MAP2, Nestin and TUJ1, and the characteristics of astrocytes were GFAP (C). iNOS (D) and Arg-1 (E) were detected by immunofluorescence after the BV2 model was established in vitro. Quantitative analysis of immunofluorescence staining (F). Expression of proinflammatory cytokines iNOS, IL-6, TNF-α (G) and anti-inflammatory cytokines Arg-1, CD206 and IL-4 (H) by PCR. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: The immunofluorescence steps of NSCs and BV2 differentiation are consistent with the identification of fibroblast, and primary antibodies were: inducible nitric oxide synthase (iNOS,6022, Proteintech, Rosemont, USA), Arginase-1 (Arg-1, 16001–1-AP, Proteintech), GFAP (3670, CST), and microtubule-associated protein 2 (MAP2, 17490–1-AP, Proteintech).

Techniques: Immunofluorescence, Staining, In Vitro, Expressing